Come see us at ELRIG in Manchester

We are exhibiting at ELRIG Drug Discovery in Manchester on 7^th and 8^th of September. ELRIG (European Laboratory and Robotics Interest Group) are running their fifth annual Drug Discovery conference this year and we will be making an appearance once again.

As in previous years the objective of this conference is to provide a communication, education and networking opportunity for scientists engaged in early-phase drug discovery whether in academia, biotech or industry. Attendees will hear world class speakers discussing the challenges and opportunities facing the drug discovery scientist. The scientific sessions will be complemented by a large poster session, a vendor exhibition and a number of training sessions.

The conference is being held at Manchester Central and we will be at Stand F2

Attend the exhibition for FREE – register here

Click here for directions on how to get there.

New Catalogues Available

Don’t forget that Abnova have a wide range of catalogues available for you to download or order.

Follow the links below to download the PDF, or alternatively email office@caltagmedsystems.co.uk to order your paper copy.

• Antibody Tools for Immunohistochemistry
• Antibody Tools for Immunofluorescence
• Antibody Tools for Immunoprecipitation
• Antibody Pairs for ELISA, IP-WB, Protein Phosphorylation, Protein-Protein Interaction
• ELISA and Assay Kits
• Fluorescence In Situ Hybridization
• Immunology
• Mitochondria

To place your catalogue order, please email office@caltagmedsystems.co.uk.

Bank Holiday Update

As we are all aware next week is a 4 day week....which means a 3 day weekend! An extra day to get those odd jobs done, do a spot of gardening, lie in after a late night or sit in the sunshine – hopefully! This means that we will be enjoying the 3 day weekend too and shutting up shop for Monday 29^th August – sorry!

So enjoy a day away from the office/lab, a day away from the commute and make good use of the long weekend.

Fingers crossed for the weather and see you Tuesday.

Symansis – Multi-Kinase ELISA Array

Symansis’ flexible Multi-Kinase Array allows you to simultaneously assay for activation of multiple kinases on a single ELISA plate giving you results within 5hours. The mix and match format allows you to assay up to 6 different kinases on a single plate, or alternatively you can just get a whole plate with a single assay. Included is the microtiter ELISA plate with 12 removable 8-well strips, of which at least two 8-well strips for any phosphokinase assay must be ordered on a plate. Every kit comes with all reagents needed to perform the assays and each strip of 8 wells contains antibodies for a specific kinase. All kit components, including the wells, are colour coordinated for ease of use.

To build-your-own Multi-Kinase ELISA Array, edit this order form to your specifications, attach it to an email and send it to http://www.blogger.com/office@caltagmedsystems.co.uk.

Product Code: MKA001

Check out the Multi-Kinase ELISA Array poster.

QuantiGene® ViewRNA ISH Cell Assay for Fluorescent RNA In Situ Hybridization (RNA FISH)

QuantiGene ViewRNA Assays are novel in situ hybridization (ISH) assays enabling localization and quantitation with single-copy RNA sensitivity at single cell resolution in cells or tissues in singleplex and multiplex assay formats.

Fluorescent based in situ hybridization (FISH) techniques are used to visualize DNA or localized RNAs within cells. However the fluorescent in situ hybridization of RNA has always been limited by low sensitivity, complicated workflow and the inability to perform multiplex analysis. QuantiGene ViewRNA is a novel non-radioactive RNA fluorescent in situ hybridization solution that offers single-copy per cell mRNA sensitivity for one to four RNA targets. Affymetrix Research-Use-Only (RUO) products for RNA FISH analysis allow investigators doing biomarker discovery or clinical research to validate and study RNA biomarkers in suspension or adherent cells.

Unique Benefits

• Sensitive in situ hybridization assay – detect one mRNA copy/cell
• Multiplex in situ hybridization – study one to four mRNA targets per cell
• Flexible probe set design – to target any mRNA sequence
• Fast results – one day assay
• Fluorescent signal – use of fluorescent microscope (no radioactivity involved)
• Robust, reproducible results in adherent or suspension cells

Applications

• Analysis of transcriptional heterogeneity
• Cancer and other disease research
• Biomarker validation
• Neurobiology/neuroscience
• Infectious diseases/virology
• Stem cells and differentiation research
• RNAi knockdown quantitation
• Study of non-coding RNAs
• An alternative to immunofluorescence (IF), immunocytochemistry (ICC) or DNA FISH assays

This assay has two required components - one assay kit and the target specific probe sets, each sold separately:

• QuantiGene ViewRNA ISH Cell Assay Kit (Product Code: QVC0001)
• QuantiGene ViewRNA Probe Sets - target specific probes (TYPE 1, 4, 6 and/or 10; download list of available probes PDF here

Download the product documentation here.

LYMPHOCLONAL for use in flow cytometry

Cytognos is a biotechnology company dedicated to the design and development of new reagents, software and techniques that provide innovative solutions in the field of flow cytometry.

LYMPHOCLONAL^TM is a four-colour direct immunofluorescence reagent for use in flow cytometry. Designed to simultaneously determine in peripheral blood, bone marrow and other body fluids the major lymphocyte subpopulations, including the total number of T lymphocytes (CD3+), B lymphocytes (CD19+) and natural killer cells (CD3-CD56+) as well as helper/inducer (CD3+CD4+) and suppressor/cytotoxic (CD3+CD8+) and T lymphocyte subsets. It can also determine B lymphocytes with immunoglobulins bearing kappa light chains (CD19+ Igκ+), and B lymphocytes with immunoglobulins bearing lambda light chains (CD19+ Igλ+).

LYMPHOCLONAL^TM recognizes the antigens CD3, CD19, CD56, CD4, CD8, kappa light chains and lambda light chains present in the different lymphocyte subsets, and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc.

To view LYMPHOCLONAL products click here.

Arbor Assays - Urea Nitrogen (BUN) Detection Kit

Product Code: K024-H1

Features:

• Precise - N-Cal™ NIST-Calibrated Kit
• Stable - Room Temperature Stable Liquid Reagents
• Economical - Complete kit for 192 Determinations
• Rapid - 30 Minute Assay
• High Optical Density - Generate > 1.7 OD for 10 mg/dL

The new Urea Nitrogen kit is a N-Cal™ kit, one of many Arbor Assay products calibrated to US National Institutes of Science and Technology (NIST) Standard Reference Materials (SRM). The calibration of these N-Cal™ kits to a SRM means you are ensured of the most accurate quantification of your samples. Urea is a by-product of protein metabolism by the liver, and is therefore removed from the blood by the kidneys. Urea freely filters through the glomerulous, but is reabsorbed by the renal tubules in a flow-dependent fashion. The higher the flow rate, the greater amount of urea nitrogen is cleared from circulation and eliminated through the kidneys. The Urea Nitrogen (BUN) Detection kit allows up to 88 samples in duplicate to be measured against the NIST-calibrated standard in 30 minutes. The kit, with its liquid reagents, is stable stored at room temperature. Two 96-well plates are provided for measurements, along with all the necessary reagents for detection.

Download product documentation here.

Download DetectX Assays for Renal Injury here.

MBL introduce MaxBlot

MaxBlot is a signal enhancer for Western Blotting and ELISA.

• Ready to Use: Primary and Secondary Antibody Diluents
• Improve signal to noise ratio by up to 10 fold
• Enhanced sensitivity of antibody-antigen reaction

Tris-buffered saline (TBS) and phosphate-buffered-saline (PBS) are standard as antibody diluents for Western Blotting (WB) and Enzyme-Linked Immunosorbent Assays (ELISA). MaxBlot is a highly optimized solution dedicated to increase the weak signals when using conventional TBS and PBS solutions for WB and ELISA. The antibody diluents formulas have been formulated to produce the most optimal antigen-antibody reaction, with a signal to noise ratio increase of up to 10 fold. To use MaxBlot, simply replace the TBS or PBS diluents with the appropriate MaxBlot solution.

MaxBlot Solution 1 optimized for primary antibodies.

MaxBlot Solution 2 optimized for secondary antibodies.

MaxBlot Solution 1 & 2 (250ml each) (Product Code: 8455)

MaxBlot Solution 1 (250ml) (Product Code: 8455-100)

MaxBlot Solution 2 (250ml) (Product Code: 8455-200)

MaxBlot Solution 1 & 2 Sample size (10ml each) (Product Code: 8455A)

Download the product datasheet here.

FIX & PERM® by An Der Grub

An Der Grub’s FIX&PERM® for suspension stainings and flow cytometric analysis of intracellular antigens.

Flow cytometric analysis with monoclonal antibodies has been primarily restricted to cell surface molecules. Intracellular structures including cytoplasmic or nuclear enzymes, oncoproteins, cytokines and immunoglobulins were largely excluded from such studies.

Also excluded from flow cytometric studies were cytoplasmic localizations of well established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM^® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies.

The FIX&PERM^® Cell Permeabilization Kit contains 2 reagents: Fixation Medium (Solution A) and Permeabilization Medium (Solution B). It is intended for first fixing cells in suspension with Solution A and then permeabilizing the cell membranes with Solution B. This procedure gives antibodies access to intracellular structures and leaves the morphological scatter characteristics of cells intact.

Specific formulations reduce background staining and allow simultaneous addition of permeabilization medium and fluorochrome labeled antibodies. FIX&PERM^® is suitable for the analysis of normal and malignant leukocyte populations derived from various human biological samples (blood, bone marrow and others) using flow cytometry.

FIX&PERM Kit (Product Code: GAS-002)

FIX&PERM Sample Kit (Product Code: GAS-002M)

FIX&PERM Kit 1000 (Product Code: GAS-002-1)

FIX&PERM Solution A (Fix) (Product Code: Gas-002A-1)

FIX&PERM Solution B (Perm) (Product Code: GAS-002B-1)

Click here to view the FIX&PERM® Brochure

AdipoGen ELISA kits...

AdipoGen have a wide range of ELISA kits including:

Progranulin – A new ligland to TNF receptors

Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signalling cascades that control cell cycle progression and cell motility. Progranulin mutations are connected to Alzheimer’s disease (AD). Progranulin detection is a potential biomarker for AD and Mild Cognitive Impairment (MCI). Progranulin binds to TNF receptors and is therapeutic against inflammatory arthritis in mice.

The AdipoGen ELISA comes in 3 sizes; 1 x 96 well plate, 2 x 96 well plates, 5 x 96 well plates.

Download the product data sheet here.

Download the AdipoGen ELISA catalogue here.

• For a quantitative and specific measurement of BAFF levels


• The first “highly sensitive” mAb/mAb-based human BAFF immunoassay


• Detects BAFF levels in patients with healthy and pathological conditions


• Secured re-supply – available from stock


• Inter- and intra- batch reproducibility


• Small original sample amounts – only 5ul needed


• Works on serum, plasma and cell culture supernatant

BAFF ELISA kit comes in one size; 1 x 96 well plate.

Download the product datasheet here.

Sirtuin 1 (Intracellular) ELISA kit

Sirtuins (Silent information regulators; SIRTs) deacetylate and/or ADP-ribosylate lysine residues of target proteins in an NAD+-dependent manner. Sirtuins are critical regulators of many cellular processes, including insulin secretion, the cell cycle and apoptosis. Sirtuins, in particular SIRT1, emerged as critical regulators of longevity and aging in several model organisms. Furthermore, a variety of age-associated diseases such as type 2 diabetes, obesity, osteoporosis, and Alzheimer’s disease are associated with sirtuins. SIRT1 has been suggested as potential biomarker for tumorigenesis.

This Adipogen ELISA comes in 2 pack sizes: 1 x 96 well plate and 2 x 96 well plates.

Download the product datasheet here.

Signosis launch new product

Signosis have launched a Multiple TF Reporter Real-time PCR Kit

Product Code: CL-2001.

Signosis has developed a proprietary technology for monitoring the activation of multiple Transcription Factors simultaneously. In TF reporter real-time PCR kit, 24 TF reporter vector mix is transfected into the cells in one transfection. The activation of 24 TFs is monitored simultaneously with SYBR green based real-time PCR directly in cell lysates without RNA preparation with CL™ cell lysis buffer.

The procedure is very simple with 3 steps:

1. TF reporter vector mix containing 24 TF reporter vectors is transfected into cells. Each TF has a corresponding cis-element and a unique reporter tag sequence.
2. The transfected cells are treated with and without an inducer for 3-5 hours.
3. The cell lysates are prepared, transcribed into cDNA and subsequently subjected to real-time PCR. The difference in TF’s can be identified through comparison of two samples.

Download the product datasheet here.

IQ's HIT Alert kit

IQ’s mission is to contribute to better healthcare by developing and providing smart, innovative and reliable diagnostics worldwide in the fields of Sepsis, Transplantation, Perinatal diagnostics and routine flow cytometry.

IQ’s featured product this week is HIT Alert - a complete kit for the reliable diagnosis of Heparin-Induced Thrombocytopenia (HIT).

HIT Alert is the first IVD/CE registered functional assay for the diagnosis of HIT in heparin-treated patients, using flow cytometry.

Special features of HIT Alert include:

• Provides rapid results (<2 hr)
• Complete kit with ready to use components
• Non-radioactive method
• Standard equipment and methodology
• Extensive evaluation - Data available
• Patented method

Product Code: IQP-396

For more information on HIT Alert download the product data sheet here.

Monosan launch new antibody

Mouse anti-Cytokeratin 5⁄6 with clone D5/16B4.

This antibody, with product code MON9125, reacts with Cytokeratin 5/6 and reacts weakly with Cytokeratin 4. Cytokeratin 5/6 are known as high molecular weights cytokeratins, and are part of the basic subfamily of cytokeratins. These two cytokeratins are closely related and are focused in non-keratizing stratified squamous epithelia, apocrine and sweat glands of skin, epidermis, hair follicles and mammalian glands.

MON9125 stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma and to differentiate epitheloid mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody to distinguish benign ductal hyperplasias from non-invasive neoplastic proliferations.

Product Code: MON9125

Uscn - now supplied by Caltag Medsystems

Uscn Life Science Inc. serves healthcare institutions, life science researchers, clinical laboratories, industry and the general public. Uscn are based in China and have a large product portfolio of over 4700 ELISA Kits for targets across a wide variety of species. Their product range also includes proteins, antibodies, CLIA Kits and other life science reagents.

The Uscn manufacturing process is based on the ability to efficiently separate and purify natural proteins from tissues or biological liquids in order to raise specific antibodies. This technology has been applied to proteins derived from plants, animals and human. Uscn also have the ability to manufacture recombinant proteins using their own genetic engineering techniques. The company possesses several patented technologies and intellectual property rights in the field of protein separation and ELISA production.

To view Uscn products click here.

Symansis - Easy 50 PI3K Inhibitor Array

This unique Easy 50^TM PI3K Inhibitor Array is a panel of serial dilutions of inhibitors that are known to specifically target the PI3K signalling pathway, and related pathways. Data produced from this array gives an inhibition profile that provides a more comprehensive understanding of the pathway. This array comes in a convenient 96 well format ready to dilute and add straight to cells.

Easy 50^TM PI3K Inhibitor Array contains 9 carefully selected cell permeable, chemically diverse and potent PI3K isoform selective inhibitors of which each is provided in a pre-titrated determined concentration range.

Product Code: IAP001

Download the poster here.

For more information or to place an order, please visit our website.

AdipoGen – now distributed by Caltag Medsystems.

Adipogen supply advanced ELISA kits for the life science reagents market including the areas of cancer, immunology, inflammation, metabolic syndrome (diabetes, obesity), stem cells and neurodegeneration.

In addition to the antibody and protein facilities, AdipoGen also own chemical laboratories, enabling them to develop new and innovative biochemicals.

The AdipoGen Institute for Life Science connects immunology to metabolism. In the next decades both diseases, obesity and diabetes, are considered to increase significantly worldwide. It becomes more and more evident that both diseases are not only a result of unbalanced nutrition but are closely related to inflammatory processes. To establish better cures of the diseases, it is necessary to understand the cross-talk between the hormones and cytokines, which are secreted from fat cells and immune cells, and to understand the principles of the specific binding of ligands to the receptors. The Adipogen Institute for Life Science offers a drug design platform, through i) discovering and characterizing adipokines and cytokines, which are secreted from fat cells and immune cells regulating glucose and fat metabolism, and ii) identifying and characterizing the receptor genes of these secreted factors.

Download the AdipoGen ELISA catalogue here.

Discount on Monosan products!

Summer offers on Monosan Xtra Products

Until the 30^th September 2011 you can get 10% discount on all Monosan Xtra products.

As known the Monosan Xtra product line is a unique addendum to the regular Monosan portfolio. The Monosan Xtra range, contains over 800 antibodies, mainly manufactured for immunohistochemical (IHC) and immunofluorescence (IF) use on frozen and FFPE tissue sections.

Monosan Xtra, monoclonal and polyclonal antibodies for research, contains products in areas like Cluster Differentiation Markers, Cytokeratins, Tumour Markers, Hormone Receptors and Estrogen Regulated Proteins and are available with 10% discount this summer.

View the full Monosan Xtra range here

30% discount on Monosan Streptavidin R-Phycoerytherin (R-PE)

R-PE (R-Phycoerythrin), a fluorescent protein, belongs to the phycobiliproteins family - a family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. R-PE consists of α, β and γ subunits and is present as (αβ)6γ. It has broad absorption bands with peaks at 565nm, 498nm and 539nm therefore can be excited with versatile excitation sources. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins and 10-20 times brighter than conventional organic fluorophores. Phycobiliproteins are also more photostable than their organic counterparts. R-PE labeled streptavidin is widely used in flow cytometry, live cell staining, and multi-color immunofluorescent staining. Monosan Streptavidin R-Phycoerytherin (R-PE) is available with product code MSA1004.

Offer valid until 30th September 2011

For more information about Monosan click here

Abnova offer wide range of kits!

Abnova have over 1000 kits on their catalogue and here at Caltag Medsystems we supply them all!

This week we’re talking about ELISA kits.

Enzyme-Linked Immunosorbent Assay (ELISA) kits are used mainly to detect and quantify proteins, antibodies, peptides, or hormones in a sample. In ELISA’s, antigens are immobilized on a solid support, either coated directly or through specific capture antibodies. Primary detection antibodies are then applied, forming a complex with the antigen. If conjugated with an enzyme, the detection antibody can directly be used to quantify the antigen, or can itself be quantified by another enzyme-conjugated secondary antibody. The method chosen depends on which target you are investigating.

Featured Abnova kits this month are the following:

• Corticosterone ELISA Kit (product code: KA0468)
• Ferritin (Human) ELISA Kit (product code: KA0211)
• Bdnf (Mouse) ELISA Kit (product code: KA0331)
• EPO (Human) ELISA Kit (product code: KA0175)
• Anti-Lactoferrin Ab ELISA Kit (product code: KA1267)
• Albumin (Mouse) ELISA Kit (product code: KA0489)
• LBP (Human) ELISA Kit (product code: KA0448)
• APOB (Human) ELISA Kit (product code: KA1028)
• PMN-Elastase (Human) ELISA Kit (product code: KA0163)

To see more ELISA kits click here.

Precipitor™ - Protein and Nucleic Acid Purification

High Throughput Immunoprecipitation and Purification

Product code: M0002

Available exclusively through Caltag Medsystems; Abnova’s Precipitor^TM systems is an automated magnetic bead platform for high throughput precipitation and purification of proteins.

Combining a 96 deep well plate with affinity conjugulated magnetic beads; Precipitor^TM easily handles 16 different assays simultaneously. It transfers beads from one well to the next for mixing, binding, washing and elution reactions via the robotic action of parallel magnetic rods.

Advantages

• Rapid, multi-step automation
• 96 well plate format
• Reproducilbe consistent results
• Multiplex analysis of up to 16 analytes
• No external device or PC required
• Open system

Applications

• Immunoprecipitation
• Chromatin-Immunoprecipitation
• RNA-Immunoprecipitation
• Recombinant Protein Purification
• Protein-Protein Interaction
• Nuclear Acid Extraction

For more information, videos and related products please visit http://www.precipitor.co.uk/

Download a related products PDF here.

Monosan announce the release of HGAL for the detection of follicular lymphoma.

This RUO antibody is available as concentrate or predilute (ready to use). Monosan’s HGAL is compatible with most automated staining platforms.

HGAL is a mouse monoclonal antibody that has the highest sensitivity of all established diagnostic markers (CD10, bcl6, and bcl2) for follicular lymphoma. It marks both follicular components and the diffuse interfollicular components that other markers are not sensitive enough to detect. CD10 and bcl6 have been more difficult antibodies to work with on lymphoid tissues, but HGAL doesn’t share this laborious characteristic. The high degree of specificity for germinal center B-cells makes anti-HGAL an ideal marker for the detection of germinal center-derived B-cell lymphomas.

benign follicle in tonsil follicular lymphoma

Moab anti HGAL, 1.0ml concentrate (product code: MON3368)

Moab anti HGAL, 7 mL pre-diluted ready-to-use (product code: MON-RTU1249)

Click here to find out more about Monosan